Subgroup Q: Next Generation Correlative Microscopy: Biological Applications and Emerging Techniques
Saturday, December 8, 1:30 pm-5:30 pm
Organizers: Ori Avinoam, Weizmann Institute of Science, Rehovot, Israel; and Yannick Schwab, European Molecular Biology Laboratory, Heidelberg Germany
Gaining a molecular level understanding of biological processes depends on developing the capability to visualize the molecules involved relative to their location within the cell at high resolution. However, obtaining such high-resolution information on dynamic processes in situ often require the application of more than one imaging technology to the exact same substrate by correlative microscopy. At the cross-road of multidisciplinary approaches, correlative microscopy brings together biologists, image analysts and engineers, leading to powerful workflows that are applied to an ever-increasing number of biological questions and models. This session will highlight the most recent advances in correlative microscopy and their application to unravel cellular organization and the molecular function of protein machineries in their subcellular context.
1:30 pm Introduction. Ori Avinoam, Weizmann Institute of Science, Rehovot, Israel, and Yannick Schwab, European Molecular Biology Laboratory, Heidelberg Germany
1:40 pm Transcytosis balances apical-basal membrane sorting during subcellular lumen formation: what do we learn from CLEM. Maria Leptin, European Molecular Biology Laboratory, Heidelberg, Germany
2:10 pm Large volume correlative light electron microscopy to obtain functional insights on endomembrane organization and its subversion by pathogens. Jost Enninga, Institut Pasteur, Paris, France
2:30 pm Life, imaged Live: monitoring early metastatic events with intravital and correlative microscopy. Matthia Karreman, Germany Cancer Research Institute (DKFZ), Heidelberg, Germany
2:50 pm Combining cryo fluorescence microscopy with cryo electron tomography to study native cellular membranes. Wanda Kukulski, MRC Laboratory of Molecular Biology, Cambridge, UK
3:10 pm Break
3:30 pm New insights to cellular composition by scanning transmission electron cryo-microscopy. Michael Elbaum, Weizmann Institute of Science, Rehovot, Israel
3:50 A multimodal imaging approach to dissect the role of endocytosis during secretion. Kamalesh Kumari, Weizmann Institute of Science, Rehovot, Israel.
4:10 Correlated cryo fluorescent and soft X-ray tomography. Carolyn Larabell, University of California San Francisco, San Francisco CA, USA.
4:30 COMULIS, a new network to bridge (pre) clinical imaging and correlative Light Electron microscopy. Focus on multidimensional registration software. Perrine Paul-Gilloteaux, CNRS France-BioImaging Nantes, France.
4:50 pm Large volume electron microscopy imaging and its correlation to cryo-fluorescence microscopy. Harald F. Hess, Janelia Research Campus,
5:20 pm Question and answer panel with all speakers
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