Subgroup L

Subgroup L: Cellular Organization of Metabolism: Biology, Structure, and Function of Enzyme Polymers

Saturday, December 8, 1:30 pm-5:30 pm
Room: 33C

Organizers: Justin Kollman, University of Washington; and Jeffrey Peterson, Fox Chase Cancer Center

An increasing number of enzymes dynamically and reversibly assemble into cellular structures in response to changes in nutrient availability or other environmental cues. These structures represent a novel non-membrane-bound mechanism for compartmentalization and localization of enzymatic activity. Many of the enzymes that undergo dynamic reorganization are metabolic enzymes (e.g., CTP synthase, inosine monophosphate dehydrogenase, and phosphofructokinase). The discovery of complex and dynamic spatial organization of enzymes opens an exciting new field at the interface between cell biology and metabolism. Most examples of dynamically reorganizing metabolic structures remain functionally uncharacterized, but the few examples that are characterized suggest that this larger scale organization plays fundamental roles in enzyme activity and for maintaining cellular homeostasis. Important questions remain for many metabolic enzyme structures: What are their mechanisms of assembly and disassembly? What is their biological function? How is assembly regulated? The presentations in this subgroup will discuss recent advances in addressing these questions, both in vitro and in vivo, for a variety of polymerizing enzymes.


1:30 pm          Emergent physical and cellular properties of synthetic, infinite protein assemblies. Emmanuel Levy, Weizmann Institute

1:50 pm          Supramolecular Assembly of Metabolic Enzymes in Saccharomyces cerevisiae. Chalongrat Noree, Mahidol University

2:10 pm          Controlling the activity of eukaryotic acetyl-CoA carboxylases: conformational locking and polymerization. Timm Maier, University of Basel

2:30 pm          Filament formation by the Phosphofructokinase-1, the gatekeeper of glycolysis. Bradley Webb, West Virginia University

2:50 pm          Metabolite regulation of stress granule composition and assembly. James Wilhelm, University of California, San Diego

3:10 pm          Histidine-mediated protein methylation and CTPS compartmentalization. Li-Mei Pai, Chang Gung University

3:30 pm          Break

3:50 pm          The structural basis for regulation of nucleotide biosynthesis enzymes by polymerization. Justin Kollman, University of Washington

4:10 pm          The assembly of the cytoophidium. Ji-Long Liu, Shanghai Tech University

4:30 pm          IMPDH assembly during T cell activation. Jeffrey Peterson, Fox Chase Cancer Center

4:50 pm          Immune response-dependent assembly of IMP dehydrogenase filaments (rods/rings structures). Edward Chan, University of Florida

5:10 pm          The role of rods and rings in neurodegeneration. Naiara Akizu, Children’s Hospital of Philadelphia


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