Subgroup K

Subgroup K: Bottom-Up Cell Biology

Saturday, December 8, 1:30 pm-5:30 pm
Room: 30D

Organizers: Daniel A. Fletcher, University of California, Berkeley; and Matthew C. Good, University of Pennsylvania

In vitro reconstitution of biological processes from their component molecular parts is a mainstay of biochemistry and has emerged over the last decade as a powerful tool in cell biology. Recent studies have shown that cell-like structures with micron-scale organization can be reconstituted from nanometer-scale parts by combining purified proteins and cytoplasmic extracts with cell-like boundary conditions. By identifying the necessary and sufficient conditions for assembly, these ‘bottom-up’ studies provide new mechanistic insight that complements more traditional ‘top-down’ cell biology. Rapid progress in micropatterning, microfluidics, and microfabrication, coupled with continued advancements in biochemistry and molecular biology, raise the possibility of creating more complete cellular reconstitutions that may one day rival the complexity of live cells.


1:30 pm                Introduction: Matt Good, University of Pennsylvania, and Dan Fletcher, University of California, Berkeley

1:35 pm                Dissecting the proofreading mechanisms of endocytosis through reconstitution. Min Wu, National University of Singapore

1:50 pm                Reconstitution of an active actin-membrane composite. Satyajit Mayor, NCBS, Bangalore

2:10 pm                Reconstitution of the dynamic steady state of actin networks. Laurent Blanchoin, BBI, Grenoble

2:30 pm                Reconstitution and biophysical study of functional kinetochores. Chip Asbury, University of Washington

2:50 pm                How cells sense micron-scale curvature using the septin cytoskeleton. Amy Gladfelter, University of North Carolina at Chapel Hill

3:10 pm                Self-organization of the bacterial cell division machinery. Martin Loose, IST, Austria

3:30 pm                Reconstituting the Molecular Mechanisms of Membrane Traffic. Jeanne Stachowiak, University of Texas

3:50 pm                Autonomously Self-regulating Giant Vesicles. Atul Parikh, University of California, Davis

4:10 pm                Buckling of epithelium growing under spherical confinement. Aurelien Roux, University of Geneva

4:30 pm                Reconstructing phase transitions and kinetic proof reading in the T cell receptor signaling system. Jay Groves, University of California, Berkeley

4:50 pm                Reconstitution of dendritic cell function. Michael Dustin, New York University

5:10 pm                Synthetic Notch circuits to direct multicellular organization. Wendell Lim, University of California, San Francisco


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